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High‐efficiency transformation of archaea by direct PCR products with its application to directed evolution of a thermostable enzyme
Hyperthermophilic archaea with unique biochemical and physiological characteristics are important organisms for fundamental research of life science and have great potential for biotechnological applications. However, low transformation efficiency of foreign DNA molecules impedes developments in gen...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936305/ https://www.ncbi.nlm.nih.gov/pubmed/32602260 http://dx.doi.org/10.1111/1751-7915.13613 |