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Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood

Keeping honeybees healthy is essential, as bees are not only important for honey production but also cross-pollination of agricultural and horticultural crops; therefore, bees have a significant economic impact worldwide. Recently, the lethal disease, the American foulbrood (AFB), caused great losse...

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Autores principales: Al-Ghamdi, Ahmad A., Al-Ghamdi, Mariam S., Ahmed, Ashraf M., Mohamed, Abdel Salam A., Shaker, Ghada H., Ansari, Mohammad Javed, Dorrah, Moataza A., Khan, Khalid Ali, Ayaad, Tahany H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938142/
https://www.ncbi.nlm.nih.gov/pubmed/33732036
http://dx.doi.org/10.1016/j.sjbs.2020.12.026
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author Al-Ghamdi, Ahmad A.
Al-Ghamdi, Mariam S.
Ahmed, Ashraf M.
Mohamed, Abdel Salam A.
Shaker, Ghada H.
Ansari, Mohammad Javed
Dorrah, Moataza A.
Khan, Khalid Ali
Ayaad, Tahany H.
author_facet Al-Ghamdi, Ahmad A.
Al-Ghamdi, Mariam S.
Ahmed, Ashraf M.
Mohamed, Abdel Salam A.
Shaker, Ghada H.
Ansari, Mohammad Javed
Dorrah, Moataza A.
Khan, Khalid Ali
Ayaad, Tahany H.
author_sort Al-Ghamdi, Ahmad A.
collection PubMed
description Keeping honeybees healthy is essential, as bees are not only important for honey production but also cross-pollination of agricultural and horticultural crops; therefore, bees have a significant economic impact worldwide. Recently, the lethal disease, the American foulbrood (AFB), caused great losses of honeybee and decline of global apiculture. Recent studies have focused on using natural insect-derived antibiotics to overcome recently emerged AFB-resistance to conventional antibiotics. In support of these studies, here we investigate the possibility of producing bee-derived anti-AFB antibiotics from an indigenous honeybee, Apis mellifera jemenitica. The immune responses of the third instar stage were first induced against the standards Micrococcus luteus and Escherichia coli compared with the indigenous Paenibacillus larvae (ksuPL5). Data indicated a strong immune response against M. luteus, E. coli and P. larvae 24 h post-P. larvae-injection as revealed by the detection of lysozyme-like, cecropin-like and prophenoloxidase (PO) activities in the plasma of P. larvae-injected third instars. Nodulation activity against injected P. larvae as early as 4 h and peaking 48 h post-P. larvae injection were observed. Potentially active anti-P. larvae immune peptide fractions purified by high-performance liquid chromatography (HPLC) showed significant in vivo therapeutic effects on P. larvae-infected first instars. Mass spectrophotometric analysis and Orbitrap measurements of P. larvae-injected plasma indicated the expression of PO (Mr: 80 kDa), beta-1,3-glucan-binding protein (Mr: 52 kDa) and serine protease 44 isoform X1 (Mr: 46 kDa). This suggests that one or all of these immune peptides contribute to significant survivorship of P. larvae-infected broods, and could be a valuable clue in the search for honeybee-derived anti-AFB natural therapeutic agents. Further molecular characterization and description of the functional roles of these predicted antimicrobial peptides from both broods and adult honeybee may enrich the arsenal of insect-derived antibiotics of therapeutic purposes.
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spelling pubmed-79381422021-03-16 Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood Al-Ghamdi, Ahmad A. Al-Ghamdi, Mariam S. Ahmed, Ashraf M. Mohamed, Abdel Salam A. Shaker, Ghada H. Ansari, Mohammad Javed Dorrah, Moataza A. Khan, Khalid Ali Ayaad, Tahany H. Saudi J Biol Sci Original Article Keeping honeybees healthy is essential, as bees are not only important for honey production but also cross-pollination of agricultural and horticultural crops; therefore, bees have a significant economic impact worldwide. Recently, the lethal disease, the American foulbrood (AFB), caused great losses of honeybee and decline of global apiculture. Recent studies have focused on using natural insect-derived antibiotics to overcome recently emerged AFB-resistance to conventional antibiotics. In support of these studies, here we investigate the possibility of producing bee-derived anti-AFB antibiotics from an indigenous honeybee, Apis mellifera jemenitica. The immune responses of the third instar stage were first induced against the standards Micrococcus luteus and Escherichia coli compared with the indigenous Paenibacillus larvae (ksuPL5). Data indicated a strong immune response against M. luteus, E. coli and P. larvae 24 h post-P. larvae-injection as revealed by the detection of lysozyme-like, cecropin-like and prophenoloxidase (PO) activities in the plasma of P. larvae-injected third instars. Nodulation activity against injected P. larvae as early as 4 h and peaking 48 h post-P. larvae injection were observed. Potentially active anti-P. larvae immune peptide fractions purified by high-performance liquid chromatography (HPLC) showed significant in vivo therapeutic effects on P. larvae-infected first instars. Mass spectrophotometric analysis and Orbitrap measurements of P. larvae-injected plasma indicated the expression of PO (Mr: 80 kDa), beta-1,3-glucan-binding protein (Mr: 52 kDa) and serine protease 44 isoform X1 (Mr: 46 kDa). This suggests that one or all of these immune peptides contribute to significant survivorship of P. larvae-infected broods, and could be a valuable clue in the search for honeybee-derived anti-AFB natural therapeutic agents. Further molecular characterization and description of the functional roles of these predicted antimicrobial peptides from both broods and adult honeybee may enrich the arsenal of insect-derived antibiotics of therapeutic purposes. Elsevier 2021-03 2020-12-19 /pmc/articles/PMC7938142/ /pubmed/33732036 http://dx.doi.org/10.1016/j.sjbs.2020.12.026 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Al-Ghamdi, Ahmad A.
Al-Ghamdi, Mariam S.
Ahmed, Ashraf M.
Mohamed, Abdel Salam A.
Shaker, Ghada H.
Ansari, Mohammad Javed
Dorrah, Moataza A.
Khan, Khalid Ali
Ayaad, Tahany H.
Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood
title Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood
title_full Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood
title_fullStr Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood
title_full_unstemmed Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood
title_short Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood
title_sort immune investigation of the honeybee apis mellifera jemenitica broods: a step toward production of a bee-derived antibiotic against the american foulbrood
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938142/
https://www.ncbi.nlm.nih.gov/pubmed/33732036
http://dx.doi.org/10.1016/j.sjbs.2020.12.026
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