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Identification and probiotic potential of lactic acid bacteria from camel milk
In the present study, a total of 80 presumed lactic acid bacteria (LAB) were isolated from camel milk. Selected LAB were identified as Lactococcus lactis (cam 12), Enterococcus lactis (cam 14) and Lactobacillus plantarum (cam 15) and their potential were tested by tolerance & de-conjugation of b...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938203/ https://www.ncbi.nlm.nih.gov/pubmed/33732048 http://dx.doi.org/10.1016/j.sjbs.2020.11.062 |
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author | Sharma, Anjali Lavania, Meeta Singh, Raghvendar Lal, Banwari |
author_facet | Sharma, Anjali Lavania, Meeta Singh, Raghvendar Lal, Banwari |
author_sort | Sharma, Anjali |
collection | PubMed |
description | In the present study, a total of 80 presumed lactic acid bacteria (LAB) were isolated from camel milk. Selected LAB were identified as Lactococcus lactis (cam 12), Enterococcus lactis (cam 14) and Lactobacillus plantarum (cam 15) and their potential were tested by tolerance & de-conjugation of bile salts, antimicrobial activity, surface hydrophobicity and adhesion potential) along with this of probiotics were evaluated for curd formation and assessed for sensory properties and syneresis. Selected LABs showed antimicrobial activity against wide range of pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus and Escherchiaia. coli). LAB (cam 12, cam 14 and cam15) were highly sceptible to chloramphenicol, vancomycin, and tetracyclin. In vitro adhesion studies with Caco-2 cells demonstrated strong adhesion activity with hydrophobicity (99%) was observed. Acute oral toxicity of E. lactis and L. plantarum showed non-toxic, non-virulent and safe for industrial application. The study provides potential LAB which may act as a substitute of functional food, synthetic feed and industrial curd formulation with in the shortest span (240 min at 28–32 °C). |
format | Online Article Text |
id | pubmed-7938203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-79382032021-03-16 Identification and probiotic potential of lactic acid bacteria from camel milk Sharma, Anjali Lavania, Meeta Singh, Raghvendar Lal, Banwari Saudi J Biol Sci Original Article In the present study, a total of 80 presumed lactic acid bacteria (LAB) were isolated from camel milk. Selected LAB were identified as Lactococcus lactis (cam 12), Enterococcus lactis (cam 14) and Lactobacillus plantarum (cam 15) and their potential were tested by tolerance & de-conjugation of bile salts, antimicrobial activity, surface hydrophobicity and adhesion potential) along with this of probiotics were evaluated for curd formation and assessed for sensory properties and syneresis. Selected LABs showed antimicrobial activity against wide range of pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus and Escherchiaia. coli). LAB (cam 12, cam 14 and cam15) were highly sceptible to chloramphenicol, vancomycin, and tetracyclin. In vitro adhesion studies with Caco-2 cells demonstrated strong adhesion activity with hydrophobicity (99%) was observed. Acute oral toxicity of E. lactis and L. plantarum showed non-toxic, non-virulent and safe for industrial application. The study provides potential LAB which may act as a substitute of functional food, synthetic feed and industrial curd formulation with in the shortest span (240 min at 28–32 °C). Elsevier 2021-03 2020-11-24 /pmc/articles/PMC7938203/ /pubmed/33732048 http://dx.doi.org/10.1016/j.sjbs.2020.11.062 Text en © 2020 Published by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Sharma, Anjali Lavania, Meeta Singh, Raghvendar Lal, Banwari Identification and probiotic potential of lactic acid bacteria from camel milk |
title | Identification and probiotic potential of lactic acid bacteria from camel milk |
title_full | Identification and probiotic potential of lactic acid bacteria from camel milk |
title_fullStr | Identification and probiotic potential of lactic acid bacteria from camel milk |
title_full_unstemmed | Identification and probiotic potential of lactic acid bacteria from camel milk |
title_short | Identification and probiotic potential of lactic acid bacteria from camel milk |
title_sort | identification and probiotic potential of lactic acid bacteria from camel milk |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938203/ https://www.ncbi.nlm.nih.gov/pubmed/33732048 http://dx.doi.org/10.1016/j.sjbs.2020.11.062 |
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