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Identification and probiotic potential of lactic acid bacteria from camel milk

In the present study, a total of 80 presumed lactic acid bacteria (LAB) were isolated from camel milk. Selected LAB were identified as Lactococcus lactis (cam 12), Enterococcus lactis (cam 14) and Lactobacillus plantarum (cam 15) and their potential were tested by tolerance & de-conjugation of b...

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Autores principales: Sharma, Anjali, Lavania, Meeta, Singh, Raghvendar, Lal, Banwari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938203/
https://www.ncbi.nlm.nih.gov/pubmed/33732048
http://dx.doi.org/10.1016/j.sjbs.2020.11.062
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author Sharma, Anjali
Lavania, Meeta
Singh, Raghvendar
Lal, Banwari
author_facet Sharma, Anjali
Lavania, Meeta
Singh, Raghvendar
Lal, Banwari
author_sort Sharma, Anjali
collection PubMed
description In the present study, a total of 80 presumed lactic acid bacteria (LAB) were isolated from camel milk. Selected LAB were identified as Lactococcus lactis (cam 12), Enterococcus lactis (cam 14) and Lactobacillus plantarum (cam 15) and their potential were tested by tolerance & de-conjugation of bile salts, antimicrobial activity, surface hydrophobicity and adhesion potential) along with this of probiotics were evaluated for curd formation and assessed for sensory properties and syneresis. Selected LABs showed antimicrobial activity against wide range of pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus and Escherchiaia. coli). LAB (cam 12, cam 14 and cam15) were highly sceptible to chloramphenicol, vancomycin, and tetracyclin. In vitro adhesion studies with Caco-2 cells demonstrated strong adhesion activity with hydrophobicity (99%) was observed. Acute oral toxicity of E. lactis and L. plantarum showed non-toxic, non-virulent and safe for industrial application. The study provides potential LAB which may act as a substitute of functional food, synthetic feed and industrial curd formulation with in the shortest span (240 min at 28–32 °C).
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spelling pubmed-79382032021-03-16 Identification and probiotic potential of lactic acid bacteria from camel milk Sharma, Anjali Lavania, Meeta Singh, Raghvendar Lal, Banwari Saudi J Biol Sci Original Article In the present study, a total of 80 presumed lactic acid bacteria (LAB) were isolated from camel milk. Selected LAB were identified as Lactococcus lactis (cam 12), Enterococcus lactis (cam 14) and Lactobacillus plantarum (cam 15) and their potential were tested by tolerance & de-conjugation of bile salts, antimicrobial activity, surface hydrophobicity and adhesion potential) along with this of probiotics were evaluated for curd formation and assessed for sensory properties and syneresis. Selected LABs showed antimicrobial activity against wide range of pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus and Escherchiaia. coli). LAB (cam 12, cam 14 and cam15) were highly sceptible to chloramphenicol, vancomycin, and tetracyclin. In vitro adhesion studies with Caco-2 cells demonstrated strong adhesion activity with hydrophobicity (99%) was observed. Acute oral toxicity of E. lactis and L. plantarum showed non-toxic, non-virulent and safe for industrial application. The study provides potential LAB which may act as a substitute of functional food, synthetic feed and industrial curd formulation with in the shortest span (240 min at 28–32 °C). Elsevier 2021-03 2020-11-24 /pmc/articles/PMC7938203/ /pubmed/33732048 http://dx.doi.org/10.1016/j.sjbs.2020.11.062 Text en © 2020 Published by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Sharma, Anjali
Lavania, Meeta
Singh, Raghvendar
Lal, Banwari
Identification and probiotic potential of lactic acid bacteria from camel milk
title Identification and probiotic potential of lactic acid bacteria from camel milk
title_full Identification and probiotic potential of lactic acid bacteria from camel milk
title_fullStr Identification and probiotic potential of lactic acid bacteria from camel milk
title_full_unstemmed Identification and probiotic potential of lactic acid bacteria from camel milk
title_short Identification and probiotic potential of lactic acid bacteria from camel milk
title_sort identification and probiotic potential of lactic acid bacteria from camel milk
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938203/
https://www.ncbi.nlm.nih.gov/pubmed/33732048
http://dx.doi.org/10.1016/j.sjbs.2020.11.062
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