Removing auto-activators from yeast-two-hybrid assays by conditional negative selection

Yeast-two-hybrid (Y2H) is widely used as a strategy to detect protein–protein interactions (PPIs). Recent advancements have made it possible to generate and analyse genome-wide PPI networks en masse by coupling Y2H with next-generation sequencing technology. However, one of the major challenges of y...

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Detalles Bibliográficos
Autores principales: Shivhare, Devendra, Musialak-Lange, Magdalena, Julca, Irene, Gluza, Pawel, Mutwil, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943551/
https://www.ncbi.nlm.nih.gov/pubmed/33750818
http://dx.doi.org/10.1038/s41598-021-84608-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943551/
https://www.ncbi.nlm.nih.gov/pubmed/33750818
http://dx.doi.org/10.1038/s41598-021-84608-9

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