Cargando…
Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model
BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent liver disease and is characterized by excessive fat accumulation. Kaili Sour Soup, a food typical of Guizhou Province, is believed to have significant health benefits. Thus, we aimed to identify and assess the impact of Kaili...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945315/ https://www.ncbi.nlm.nih.gov/pubmed/33691721 http://dx.doi.org/10.1186/s12986-021-00553-4 |
_version_ | 1783662839627513856 |
---|---|
author | Cong, Shuo Li, Zhengchao Yu, Lei Liu, Yongmei Hu, Yaxin Bi, Ying Cheng, Mingliang |
author_facet | Cong, Shuo Li, Zhengchao Yu, Lei Liu, Yongmei Hu, Yaxin Bi, Ying Cheng, Mingliang |
author_sort | Cong, Shuo |
collection | PubMed |
description | BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent liver disease and is characterized by excessive fat accumulation. Kaili Sour Soup, a food typical of Guizhou Province, is believed to have significant health benefits. Thus, we aimed to identify and assess the impact of Kaili Sour Soup on NAFLD and its underlying mechanism using integrative proteomic and lipidomic analysis. METHODS: A high-fat diet and male Wistar rats were used to construct a NAFLD rat model. Haematoxylin and eosin (HE) and Oil Red O staining analyses were used to perform the histologic examination. Proteomic analysis was utilized to systematically identify the global protein profile in NAFLD with and without Kaili Sour Soup treatment. Western blot assays were used to verify the expression of proteins screened by proteomic analysis. Lipidomic analysis was performed to screen lipid metabolism in NAFLD with and without Kaili Sour Soup treatment. RESULTS: Kaili Sour Soup alleviated high-fat diet (HFD)-induced fatty liver and had a normalizing effect on physiological and biochemical indicators of NAFLD, including body weight, liver weight, liver index, total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and insulin resistance level of homeostasis model assessment (HOMA-IR). Kaili Sour Soup decreased the levels of 13 proteins (Tmem44, Rnaseh2b, Gstm6l, LOC100910877, Rufy4, Slc12a2, Pcif1, P4503A1, Sult1e1, Nop53, AABR07065656.4, AABR07065789.3) that were upregulated by HFD and increased the levels of 3 proteins (Sult1c2, Sult1c2a, Snrnp48) that were downregulated by HFD. Kaili Sour Soup attenuated the HFD-induced increase in acyl carnitine (AcCa) and enhanced the HFD-induced decreases in gangliosides (GM3) and lysophosphatidylserine (LPS) in the NAFLD rat model. CONCLUSIONS: Altogether, this study revealed that Kaili Sour Soup attenuated HFD-induced fatty liver and systematically identified abnormal proteins and lipids involved in the role of Kaili Sour Soup in a NAFLD rat model. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-021-00553-4. |
format | Online Article Text |
id | pubmed-7945315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79453152021-03-10 Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model Cong, Shuo Li, Zhengchao Yu, Lei Liu, Yongmei Hu, Yaxin Bi, Ying Cheng, Mingliang Nutr Metab (Lond) Research BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent liver disease and is characterized by excessive fat accumulation. Kaili Sour Soup, a food typical of Guizhou Province, is believed to have significant health benefits. Thus, we aimed to identify and assess the impact of Kaili Sour Soup on NAFLD and its underlying mechanism using integrative proteomic and lipidomic analysis. METHODS: A high-fat diet and male Wistar rats were used to construct a NAFLD rat model. Haematoxylin and eosin (HE) and Oil Red O staining analyses were used to perform the histologic examination. Proteomic analysis was utilized to systematically identify the global protein profile in NAFLD with and without Kaili Sour Soup treatment. Western blot assays were used to verify the expression of proteins screened by proteomic analysis. Lipidomic analysis was performed to screen lipid metabolism in NAFLD with and without Kaili Sour Soup treatment. RESULTS: Kaili Sour Soup alleviated high-fat diet (HFD)-induced fatty liver and had a normalizing effect on physiological and biochemical indicators of NAFLD, including body weight, liver weight, liver index, total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and insulin resistance level of homeostasis model assessment (HOMA-IR). Kaili Sour Soup decreased the levels of 13 proteins (Tmem44, Rnaseh2b, Gstm6l, LOC100910877, Rufy4, Slc12a2, Pcif1, P4503A1, Sult1e1, Nop53, AABR07065656.4, AABR07065789.3) that were upregulated by HFD and increased the levels of 3 proteins (Sult1c2, Sult1c2a, Snrnp48) that were downregulated by HFD. Kaili Sour Soup attenuated the HFD-induced increase in acyl carnitine (AcCa) and enhanced the HFD-induced decreases in gangliosides (GM3) and lysophosphatidylserine (LPS) in the NAFLD rat model. CONCLUSIONS: Altogether, this study revealed that Kaili Sour Soup attenuated HFD-induced fatty liver and systematically identified abnormal proteins and lipids involved in the role of Kaili Sour Soup in a NAFLD rat model. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-021-00553-4. BioMed Central 2021-03-10 /pmc/articles/PMC7945315/ /pubmed/33691721 http://dx.doi.org/10.1186/s12986-021-00553-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Cong, Shuo Li, Zhengchao Yu, Lei Liu, Yongmei Hu, Yaxin Bi, Ying Cheng, Mingliang Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model |
title | Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model |
title_full | Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model |
title_fullStr | Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model |
title_full_unstemmed | Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model |
title_short | Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model |
title_sort | integrative proteomic and lipidomic analysis of kaili sour soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945315/ https://www.ncbi.nlm.nih.gov/pubmed/33691721 http://dx.doi.org/10.1186/s12986-021-00553-4 |
work_keys_str_mv | AT congshuo integrativeproteomicandlipidomicanalysisofkailisoursoupmediatedattenuationofhighfatdietinducednonalcoholicfattyliverdiseaseinaratmodel AT lizhengchao integrativeproteomicandlipidomicanalysisofkailisoursoupmediatedattenuationofhighfatdietinducednonalcoholicfattyliverdiseaseinaratmodel AT yulei integrativeproteomicandlipidomicanalysisofkailisoursoupmediatedattenuationofhighfatdietinducednonalcoholicfattyliverdiseaseinaratmodel AT liuyongmei integrativeproteomicandlipidomicanalysisofkailisoursoupmediatedattenuationofhighfatdietinducednonalcoholicfattyliverdiseaseinaratmodel AT huyaxin integrativeproteomicandlipidomicanalysisofkailisoursoupmediatedattenuationofhighfatdietinducednonalcoholicfattyliverdiseaseinaratmodel AT biying integrativeproteomicandlipidomicanalysisofkailisoursoupmediatedattenuationofhighfatdietinducednonalcoholicfattyliverdiseaseinaratmodel AT chengmingliang integrativeproteomicandlipidomicanalysisofkailisoursoupmediatedattenuationofhighfatdietinducednonalcoholicfattyliverdiseaseinaratmodel |