Cargando…
Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2
Pseudoviruses are useful surrogates for highly pathogenic viruses because of their safety, genetic stability, and scalability for screening assays. Many different pseudovirus platforms exist, each with different advantages and limitations. Here we report our efforts to optimize and characterize an H...
Autores principales: | Neerukonda, Sabari Nath, Vassell, Russell, Herrup, Rachel, Liu, Shufeng, Wang, Tony, Takeda, Kazuyo, Yang, Ye, Lin, Tsai-Lien, Wang, Wei, Weiss, Carol D. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946320/ https://www.ncbi.nlm.nih.gov/pubmed/33690649 http://dx.doi.org/10.1371/journal.pone.0248348 |
Ejemplares similares
-
Neutralizing Antibodies Targeting the Conserved Stem Region of Influenza Hemagglutinin
por: Nath Neerukonda, Sabari, et al.
Publicado: (2020) -
Characterization of Entry Pathways, Species-Specific Angiotensin-Converting Enzyme 2 Residues Determining Entry, and Antibody Neutralization Evasion of Omicron BA.1, BA.1.1, BA.2, and BA.3 Variants
por: Neerukonda, Sabari Nath, et al.
Publicado: (2022) -
SARS-CoV-2 Delta Variant Displays Moderate Resistance to Neutralizing Antibodies and Spike Protein Properties of Higher Soluble ACE2 Sensitivity, Enhanced Cleavage and Fusogenic Activity
por: Neerukonda, Sabari Nath, et al.
Publicado: (2021) -
Effects of N-glycan modifications on spike expression, virus infectivity, and neutralization sensitivity in ancestral compared to Omicron SARS-CoV-2 variants
por: Lusvarghi, Sabrina, et al.
Publicado: (2023) -
Key Substitutions in the Spike Protein of SARS-CoV-2 Variants Can Predict Resistance to Monoclonal Antibodies, but Other Substitutions Can Modify the Effects
por: Lusvarghi, Sabrina, et al.
Publicado: (2022)