Kinetic and thermodynamic analysis defines roles for two metal ions in DNA polymerase specificity and catalysis

Magnesium ions play a critical role in catalysis by many enzymes and contribute to the fidelity of DNA polymerases through a two-metal ion mechanism. However, specificity is a kinetic phenomenon and the roles of Mg(2+) ions in each step in the catalysis have not been resolved. We first examined the...

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Detalles Bibliográficos
Autores principales: Gong, Shanzhong, Kirmizialtin, Serdal, Chang, Adrienne, Mayfield, Joshua E., Zhang, Yan Jessie, Johnson, Kenneth A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948414/
https://www.ncbi.nlm.nih.gov/pubmed/33310704
http://dx.doi.org/10.1074/jbc.RA120.016489

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948414/
https://www.ncbi.nlm.nih.gov/pubmed/33310704
http://dx.doi.org/10.1074/jbc.RA120.016489

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