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A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase

Reliable measurement of ligand binding to cell surface receptors is of outstanding biological and pharmacological importance. Resonance energy transfer–based assays are powerful approaches to achieve this goal, but the currently available methods are hindered by the necessity of receptor tagging, wh...

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Detalles Bibliográficos
Autores principales: Tóth, András Dávid, Garger, Dániel, Prokop, Susanne, Soltész-Katona, Eszter, Várnai, Péter, Balla, András, Turu, Gábor, Hunyady, László
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950324/
https://www.ncbi.nlm.nih.gov/pubmed/33545176
http://dx.doi.org/10.1016/j.jbc.2021.100366