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A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase
Reliable measurement of ligand binding to cell surface receptors is of outstanding biological and pharmacological importance. Resonance energy transfer–based assays are powerful approaches to achieve this goal, but the currently available methods are hindered by the necessity of receptor tagging, wh...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950324/ https://www.ncbi.nlm.nih.gov/pubmed/33545176 http://dx.doi.org/10.1016/j.jbc.2021.100366 |