Equivolumetric Protocol Generates Library Sizes Proportional to Total Microbial Load in 16S Amplicon Sequencing

High-throughput sequencing of 16S rRNA amplicon has been extensively employed to perform microbiome characterization worldwide. As a culture-independent methodology, it has allowed high-level profiling of sample bacterial composition directly from samples. However, most studies are limited to inform...

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Detalles Bibliográficos
Autores principales: Cruz, Giuliano Netto Flores, Christoff, Ana Paula, de Oliveira, Luiz Felipe Valter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952455/
https://www.ncbi.nlm.nih.gov/pubmed/33717032
http://dx.doi.org/10.3389/fmicb.2021.638231

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952455/
https://www.ncbi.nlm.nih.gov/pubmed/33717032
http://dx.doi.org/10.3389/fmicb.2021.638231

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