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Equivolumetric Protocol Generates Library Sizes Proportional to Total Microbial Load in 16S Amplicon Sequencing
High-throughput sequencing of 16S rRNA amplicon has been extensively employed to perform microbiome characterization worldwide. As a culture-independent methodology, it has allowed high-level profiling of sample bacterial composition directly from samples. However, most studies are limited to inform...
Autores principales: | Cruz, Giuliano Netto Flores, Christoff, Ana Paula, de Oliveira, Luiz Felipe Valter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952455/ https://www.ncbi.nlm.nih.gov/pubmed/33717032 http://dx.doi.org/10.3389/fmicb.2021.638231 |
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