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An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment

OBJECTIVE: To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with (125)I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. METHODS: The binding fraction of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells was measured...

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Autores principales: Yu, Mingming, Sun, Yanqin, Yang, Guangjie, Wang, Zhenguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953584/
https://www.ncbi.nlm.nih.gov/pubmed/33746628
http://dx.doi.org/10.1155/2021/5565932
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author Yu, Mingming
Sun, Yanqin
Yang, Guangjie
Wang, Zhenguang
author_facet Yu, Mingming
Sun, Yanqin
Yang, Guangjie
Wang, Zhenguang
author_sort Yu, Mingming
collection PubMed
description OBJECTIVE: To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with (125)I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. METHODS: The binding fraction of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. RESULTS: At pH = 6.0, the binding fractions of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1.9 ± 0.1%, 3.5 ± 0.1%, 6.3 ± 0.8%, and 6.6 ± 0.3%, respectively. At pH = 7.4, there was no measured binding between [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. CONCLUSIONS: [(125)I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection.
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spelling pubmed-79535842021-03-18 An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment Yu, Mingming Sun, Yanqin Yang, Guangjie Wang, Zhenguang Mol Imaging Research Article OBJECTIVE: To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with (125)I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. METHODS: The binding fraction of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. RESULTS: At pH = 6.0, the binding fractions of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1.9 ± 0.1%, 3.5 ± 0.1%, 6.3 ± 0.8%, and 6.6 ± 0.3%, respectively. At pH = 7.4, there was no measured binding between [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. CONCLUSIONS: [(125)I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection. Hindawi 2021-02-16 /pmc/articles/PMC7953584/ /pubmed/33746628 http://dx.doi.org/10.1155/2021/5565932 Text en Copyright © 2021 Mingming Yu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Mingming
Sun, Yanqin
Yang, Guangjie
Wang, Zhenguang
An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
title An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
title_full An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
title_fullStr An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
title_full_unstemmed An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
title_short An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
title_sort experimental study on [(125)i]i-phlip (var7) for spect/ct imaging of an mda-mb-231 triple-negative breast cancer mouse model by targeting the tumor microenvironment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953584/
https://www.ncbi.nlm.nih.gov/pubmed/33746628
http://dx.doi.org/10.1155/2021/5565932
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