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An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
OBJECTIVE: To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with (125)I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. METHODS: The binding fraction of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells was measured...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953584/ https://www.ncbi.nlm.nih.gov/pubmed/33746628 http://dx.doi.org/10.1155/2021/5565932 |
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author | Yu, Mingming Sun, Yanqin Yang, Guangjie Wang, Zhenguang |
author_facet | Yu, Mingming Sun, Yanqin Yang, Guangjie Wang, Zhenguang |
author_sort | Yu, Mingming |
collection | PubMed |
description | OBJECTIVE: To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with (125)I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. METHODS: The binding fraction of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. RESULTS: At pH = 6.0, the binding fractions of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1.9 ± 0.1%, 3.5 ± 0.1%, 6.3 ± 0.8%, and 6.6 ± 0.3%, respectively. At pH = 7.4, there was no measured binding between [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. CONCLUSIONS: [(125)I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection. |
format | Online Article Text |
id | pubmed-7953584 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-79535842021-03-18 An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment Yu, Mingming Sun, Yanqin Yang, Guangjie Wang, Zhenguang Mol Imaging Research Article OBJECTIVE: To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with (125)I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. METHODS: The binding fraction of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. RESULTS: At pH = 6.0, the binding fractions of [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1.9 ± 0.1%, 3.5 ± 0.1%, 6.3 ± 0.8%, and 6.6 ± 0.3%, respectively. At pH = 7.4, there was no measured binding between [(125)I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. CONCLUSIONS: [(125)I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection. Hindawi 2021-02-16 /pmc/articles/PMC7953584/ /pubmed/33746628 http://dx.doi.org/10.1155/2021/5565932 Text en Copyright © 2021 Mingming Yu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yu, Mingming Sun, Yanqin Yang, Guangjie Wang, Zhenguang An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
title | An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
title_full | An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
title_fullStr | An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
title_full_unstemmed | An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
title_short | An Experimental Study on [(125)I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
title_sort | experimental study on [(125)i]i-phlip (var7) for spect/ct imaging of an mda-mb-231 triple-negative breast cancer mouse model by targeting the tumor microenvironment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953584/ https://www.ncbi.nlm.nih.gov/pubmed/33746628 http://dx.doi.org/10.1155/2021/5565932 |
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