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Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency

Meiotic chromosomes have a loop/axis architecture, with axis length determining crossover frequency. Meiosis-specific Pds5 depletion mutants have shorter chromosome axes and lower homologous chromosome pairing and recombination frequency. However, it is poorly understood how Pds5 coordinately regula...

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Autores principales: Song, Meihui, Zhai, Binyuan, Yang, Xiao, Tan, Taicong, Wang, Ying, Yang, Xuan, Tan, Yingjin, Chu, Tingting, Cao, Yanding, Song, Yulong, Wang, Shunxin, Zhang, Liangran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954452/
https://www.ncbi.nlm.nih.gov/pubmed/33712462
http://dx.doi.org/10.1126/sciadv.abe7920
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author Song, Meihui
Zhai, Binyuan
Yang, Xiao
Tan, Taicong
Wang, Ying
Yang, Xuan
Tan, Yingjin
Chu, Tingting
Cao, Yanding
Song, Yulong
Wang, Shunxin
Zhang, Liangran
author_facet Song, Meihui
Zhai, Binyuan
Yang, Xiao
Tan, Taicong
Wang, Ying
Yang, Xuan
Tan, Yingjin
Chu, Tingting
Cao, Yanding
Song, Yulong
Wang, Shunxin
Zhang, Liangran
author_sort Song, Meihui
collection PubMed
description Meiotic chromosomes have a loop/axis architecture, with axis length determining crossover frequency. Meiosis-specific Pds5 depletion mutants have shorter chromosome axes and lower homologous chromosome pairing and recombination frequency. However, it is poorly understood how Pds5 coordinately regulates these processes. In this study, we show that only ~20% of wild-type level of Pds5 is required for homolog pairing and that higher levels of Pds5 dosage-dependently regulate axis length and crossover frequency. Moderate changes in Pds5 protein levels do not explicitly impair the basic recombination process. Further investigations show that Pds5 does not regulate chromosome axes by altering Rec8 abundance. Conversely, Rec8 regulates chromosome axis length by modulating Pds5. These findings highlight the important role of Pds5 in regulating meiosis and its relationship with Rec8 to regulate chromosome axis length and crossover frequency with implications for evolutionary adaptation.
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spelling pubmed-79544522021-03-24 Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency Song, Meihui Zhai, Binyuan Yang, Xiao Tan, Taicong Wang, Ying Yang, Xuan Tan, Yingjin Chu, Tingting Cao, Yanding Song, Yulong Wang, Shunxin Zhang, Liangran Sci Adv Research Articles Meiotic chromosomes have a loop/axis architecture, with axis length determining crossover frequency. Meiosis-specific Pds5 depletion mutants have shorter chromosome axes and lower homologous chromosome pairing and recombination frequency. However, it is poorly understood how Pds5 coordinately regulates these processes. In this study, we show that only ~20% of wild-type level of Pds5 is required for homolog pairing and that higher levels of Pds5 dosage-dependently regulate axis length and crossover frequency. Moderate changes in Pds5 protein levels do not explicitly impair the basic recombination process. Further investigations show that Pds5 does not regulate chromosome axes by altering Rec8 abundance. Conversely, Rec8 regulates chromosome axis length by modulating Pds5. These findings highlight the important role of Pds5 in regulating meiosis and its relationship with Rec8 to regulate chromosome axis length and crossover frequency with implications for evolutionary adaptation. American Association for the Advancement of Science 2021-03-12 /pmc/articles/PMC7954452/ /pubmed/33712462 http://dx.doi.org/10.1126/sciadv.abe7920 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Song, Meihui
Zhai, Binyuan
Yang, Xiao
Tan, Taicong
Wang, Ying
Yang, Xuan
Tan, Yingjin
Chu, Tingting
Cao, Yanding
Song, Yulong
Wang, Shunxin
Zhang, Liangran
Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency
title Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency
title_full Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency
title_fullStr Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency
title_full_unstemmed Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency
title_short Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency
title_sort interplay between pds5 and rec8 in regulating chromosome axis length and crossover frequency
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954452/
https://www.ncbi.nlm.nih.gov/pubmed/33712462
http://dx.doi.org/10.1126/sciadv.abe7920
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