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Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS
Current proteomics approaches disassemble and digest nucleosome particles, blurring the readout of the “histone code”. To preserve nucleosome-level information, we developed Nuc-MS which displays the landscape of histone variants and their PTMs in a single mass spectrum. Combined with immunoprecipit...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954958/ https://www.ncbi.nlm.nih.gov/pubmed/33589837 http://dx.doi.org/10.1038/s41592-020-01052-9 |
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| author | Schachner, Luis F. Jooβ, Kevin Morgan, Marc A. Piunti, Andrea Meiners, Matthew J. Kafader, Jared O. Lee, Alexander S. Iwanaszko, Marta Cheek, Marcus A. Burg, Jonathan M. Howard, Sarah A. Keogh, Michael-Christopher Shilatifard, Ali Kelleher, Neil L. |
| author_facet | Schachner, Luis F. Jooβ, Kevin Morgan, Marc A. Piunti, Andrea Meiners, Matthew J. Kafader, Jared O. Lee, Alexander S. Iwanaszko, Marta Cheek, Marcus A. Burg, Jonathan M. Howard, Sarah A. Keogh, Michael-Christopher Shilatifard, Ali Kelleher, Neil L. |
| author_sort | Schachner, Luis F. |
| collection | PubMed |
| description | Current proteomics approaches disassemble and digest nucleosome particles, blurring the readout of the “histone code”. To preserve nucleosome-level information, we developed Nuc-MS which displays the landscape of histone variants and their PTMs in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (6-fold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., a >15-fold enrichment of H3K79me2). Nuc-MS is highly concordant with ChIP-seq and offers a new readout of nucleosome-level biology. |
| format | Online Article Text |
| id | pubmed-7954958 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-79549582021-08-15 Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS Schachner, Luis F. Jooβ, Kevin Morgan, Marc A. Piunti, Andrea Meiners, Matthew J. Kafader, Jared O. Lee, Alexander S. Iwanaszko, Marta Cheek, Marcus A. Burg, Jonathan M. Howard, Sarah A. Keogh, Michael-Christopher Shilatifard, Ali Kelleher, Neil L. Nat Methods Article Current proteomics approaches disassemble and digest nucleosome particles, blurring the readout of the “histone code”. To preserve nucleosome-level information, we developed Nuc-MS which displays the landscape of histone variants and their PTMs in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (6-fold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., a >15-fold enrichment of H3K79me2). Nuc-MS is highly concordant with ChIP-seq and offers a new readout of nucleosome-level biology. 2021-02-15 2021-03 /pmc/articles/PMC7954958/ /pubmed/33589837 http://dx.doi.org/10.1038/s41592-020-01052-9 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Schachner, Luis F. Jooβ, Kevin Morgan, Marc A. Piunti, Andrea Meiners, Matthew J. Kafader, Jared O. Lee, Alexander S. Iwanaszko, Marta Cheek, Marcus A. Burg, Jonathan M. Howard, Sarah A. Keogh, Michael-Christopher Shilatifard, Ali Kelleher, Neil L. Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS |
| title | Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS |
| title_full | Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS |
| title_fullStr | Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS |
| title_full_unstemmed | Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS |
| title_short | Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS |
| title_sort | decoding the protein composition of whole nucleosomes with nuc-ms |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954958/ https://www.ncbi.nlm.nih.gov/pubmed/33589837 http://dx.doi.org/10.1038/s41592-020-01052-9 |
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