Purification of mouse hepatic non-parenchymal cells or nuclei for use in ChIP-seq and other next-generation sequencing approaches

Significant advancements in understanding disease mechanisms can occur through combined analysis of next-generation sequencing datasets generated using purified cell populations. Here, we detail our optimized protocol for purification of mouse hepatic macrophages (or other liver non-parenchymal popu...

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Detalles Bibliográficos
Autores principales: Troutman, Ty D., Bennett, Hunter, Sakai, Mashito, Seidman, Jason S., Heinz, Sven, Glass, Christopher K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960533/
https://www.ncbi.nlm.nih.gov/pubmed/33748781
http://dx.doi.org/10.1016/j.xpro.2021.100363

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960533/
https://www.ncbi.nlm.nih.gov/pubmed/33748781
http://dx.doi.org/10.1016/j.xpro.2021.100363

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