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Purification of mouse hepatic non-parenchymal cells or nuclei for use in ChIP-seq and other next-generation sequencing approaches
Significant advancements in understanding disease mechanisms can occur through combined analysis of next-generation sequencing datasets generated using purified cell populations. Here, we detail our optimized protocol for purification of mouse hepatic macrophages (or other liver non-parenchymal popu...
Autores principales: | Troutman, Ty D., Bennett, Hunter, Sakai, Mashito, Seidman, Jason S., Heinz, Sven, Glass, Christopher K. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960533/ https://www.ncbi.nlm.nih.gov/pubmed/33748781 http://dx.doi.org/10.1016/j.xpro.2021.100363 |
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