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Conjoint expression and purification strategy for acquiring proteins with ultra-low DNA N(6)-methyladenine backgrounds in Escherichia coli
DNA N(6)-methyladenine (6mA), a kind of DNA epigenetic modification, is widespread in eukaryotes and prokaryotes. An enzyme activity study coupled with 6mA detection using ultra-high-performance liquid chromatography-quadruple mass spectrometry (UHPLC-MS/MS) is commonly applied to investigate 6mA po...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Portland Press Ltd.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960888/ https://www.ncbi.nlm.nih.gov/pubmed/33660764 http://dx.doi.org/10.1042/BSR20203769 |