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Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly

BACKGROUND: The ability to clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. The recent development of seamless cloning technologies has made significant improvements in plasmid construction, but simple and reliable tools are always desirable for tim...

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Detalles Bibliográficos
Autores principales: Chen, Fan, Li, Yi-ya, Yu, Yan-li, Dai, Jie, Huang, Jin-ling, Lin, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962268/
https://www.ncbi.nlm.nih.gov/pubmed/33722223
http://dx.doi.org/10.1186/s12896-021-00679-6