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Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly
BACKGROUND: The ability to clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. The recent development of seamless cloning technologies has made significant improvements in plasmid construction, but simple and reliable tools are always desirable for tim...
Autores principales: | Chen, Fan, Li, Yi-ya, Yu, Yan-li, Dai, Jie, Huang, Jin-ling, Lin, Jie |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962268/ https://www.ncbi.nlm.nih.gov/pubmed/33722223 http://dx.doi.org/10.1186/s12896-021-00679-6 |
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