Optimization of a bacterial three-hybrid assay through in vivo titration of an RNA–DNA adapter protein

Noncoding RNAs regulate gene expression in every domain of life. In bacteria, small RNAs (sRNAs) regulate gene expression in response to stress and are often assisted by RNA–chaperone proteins, such as Hfq. We have recently developed a bacterial three-hybrid (B3H) assay that detects the strong bindi...

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Detalles Bibliográficos
Autores principales: Wang, Clara D., Mansky, Rachel, LeBlanc, Hannah, Gravel, Chandra M., Berry, Katherine E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962490/
https://www.ncbi.nlm.nih.gov/pubmed/33500316
http://dx.doi.org/10.1261/rna.077404.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962490/
https://www.ncbi.nlm.nih.gov/pubmed/33500316
http://dx.doi.org/10.1261/rna.077404.120

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