Cargando…
Optimization of a bacterial three-hybrid assay through in vivo titration of an RNA–DNA adapter protein
Noncoding RNAs regulate gene expression in every domain of life. In bacteria, small RNAs (sRNAs) regulate gene expression in response to stress and are often assisted by RNA–chaperone proteins, such as Hfq. We have recently developed a bacterial three-hybrid (B3H) assay that detects the strong bindi...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962490/ https://www.ncbi.nlm.nih.gov/pubmed/33500316 http://dx.doi.org/10.1261/rna.077404.120 |