dCas9 regulator to neutralize competition in CRISPRi circuits

CRISPRi-mediated gene regulation allows simultaneous control of many genes. However, highly specific sgRNA-promoter binding is, alone, insufficient to achieve independent transcriptional regulation of multiple targets. Indeed, due to competition for dCas9, the repression ability of one sgRNA changes...

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Detalles Bibliográficos
Autores principales: Huang, Hsin-Ho, Bellato, Massimo, Qian, Yili, Cárdenas, Pablo, Pasotti, Lorenzo, Magni, Paolo, Del Vecchio, Domitilla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966764/
https://www.ncbi.nlm.nih.gov/pubmed/33727557
http://dx.doi.org/10.1038/s41467-021-21772-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966764/
https://www.ncbi.nlm.nih.gov/pubmed/33727557
http://dx.doi.org/10.1038/s41467-021-21772-6

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