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Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet

Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NC...

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Autores principales: Taki, Keigo, Takagi, Hiroshi, Hirose, Tomonori, Sun, Runan, Yaginuma, Hiroshi, Mizoguchi, Akira, Kobayashi, Tomoko, Sugiyama, Mariko, Tsunekawa, Taku, Onoue, Takeshi, Hagiwara, Daisuke, Ito, Yoshihiro, Iwama, Shintaro, Suga, Hidetaka, Banno, Ryoichi, Sakano, Daisuke, Kume, Shoen, Arima, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968668/
https://www.ncbi.nlm.nih.gov/pubmed/33730054
http://dx.doi.org/10.1371/journal.pone.0248065
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author Taki, Keigo
Takagi, Hiroshi
Hirose, Tomonori
Sun, Runan
Yaginuma, Hiroshi
Mizoguchi, Akira
Kobayashi, Tomoko
Sugiyama, Mariko
Tsunekawa, Taku
Onoue, Takeshi
Hagiwara, Daisuke
Ito, Yoshihiro
Iwama, Shintaro
Suga, Hidetaka
Banno, Ryoichi
Sakano, Daisuke
Kume, Shoen
Arima, Hiroshi
author_facet Taki, Keigo
Takagi, Hiroshi
Hirose, Tomonori
Sun, Runan
Yaginuma, Hiroshi
Mizoguchi, Akira
Kobayashi, Tomoko
Sugiyama, Mariko
Tsunekawa, Taku
Onoue, Takeshi
Hagiwara, Daisuke
Ito, Yoshihiro
Iwama, Shintaro
Suga, Hidetaka
Banno, Ryoichi
Sakano, Daisuke
Kume, Shoen
Arima, Hiroshi
author_sort Taki, Keigo
collection PubMed
description Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NCD plus high-NaCl diet (NCD plus NaCl), a HFD, or a HFD plus high-NaCl diet (HFD plus NaCl) for 30 weeks. No significant differences in body weight gain, insulin sensitivity, and glucose tolerance were observed between NCD-fed and NCD plus NaCl-fed mice. In contrast, body and liver weights were decreased, but the weight of epididymal white adipose tissue was increased in HFD plus NaCl-fed compared to HFD-fed mice. HFD plus NaCl-fed mice had lower plasma glucose levels in an insulin tolerance test, and showed higher plasma glucose and lower plasma insulin levels in an intraperitoneal glucose tolerance test compared to HFD-fed mice. The β-cell area and number of islets were decreased in HFD plus NaCl-fed compared to HFD-fed mice. Increased Ki67-positive β-cells, and increased expression levels of Ki67, CyclinB1, and CyclinD1 mRNA in islets were observed in HFD-fed but not HFD plus NaCl-fed mice when compared to NCD-fed mice. Our data suggest that excessive NaCl intake accompanied by a HFD exacerbates glucose intolerance, with impairment in insulin secretion caused by the attenuation of expansion of β-cell mass in the pancreas.
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spelling pubmed-79686682021-03-31 Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet Taki, Keigo Takagi, Hiroshi Hirose, Tomonori Sun, Runan Yaginuma, Hiroshi Mizoguchi, Akira Kobayashi, Tomoko Sugiyama, Mariko Tsunekawa, Taku Onoue, Takeshi Hagiwara, Daisuke Ito, Yoshihiro Iwama, Shintaro Suga, Hidetaka Banno, Ryoichi Sakano, Daisuke Kume, Shoen Arima, Hiroshi PLoS One Research Article Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NCD plus high-NaCl diet (NCD plus NaCl), a HFD, or a HFD plus high-NaCl diet (HFD plus NaCl) for 30 weeks. No significant differences in body weight gain, insulin sensitivity, and glucose tolerance were observed between NCD-fed and NCD plus NaCl-fed mice. In contrast, body and liver weights were decreased, but the weight of epididymal white adipose tissue was increased in HFD plus NaCl-fed compared to HFD-fed mice. HFD plus NaCl-fed mice had lower plasma glucose levels in an insulin tolerance test, and showed higher plasma glucose and lower plasma insulin levels in an intraperitoneal glucose tolerance test compared to HFD-fed mice. The β-cell area and number of islets were decreased in HFD plus NaCl-fed compared to HFD-fed mice. Increased Ki67-positive β-cells, and increased expression levels of Ki67, CyclinB1, and CyclinD1 mRNA in islets were observed in HFD-fed but not HFD plus NaCl-fed mice when compared to NCD-fed mice. Our data suggest that excessive NaCl intake accompanied by a HFD exacerbates glucose intolerance, with impairment in insulin secretion caused by the attenuation of expansion of β-cell mass in the pancreas. Public Library of Science 2021-03-17 /pmc/articles/PMC7968668/ /pubmed/33730054 http://dx.doi.org/10.1371/journal.pone.0248065 Text en © 2021 Taki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Taki, Keigo
Takagi, Hiroshi
Hirose, Tomonori
Sun, Runan
Yaginuma, Hiroshi
Mizoguchi, Akira
Kobayashi, Tomoko
Sugiyama, Mariko
Tsunekawa, Taku
Onoue, Takeshi
Hagiwara, Daisuke
Ito, Yoshihiro
Iwama, Shintaro
Suga, Hidetaka
Banno, Ryoichi
Sakano, Daisuke
Kume, Shoen
Arima, Hiroshi
Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
title Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
title_full Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
title_fullStr Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
title_full_unstemmed Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
title_short Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
title_sort dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968668/
https://www.ncbi.nlm.nih.gov/pubmed/33730054
http://dx.doi.org/10.1371/journal.pone.0248065
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