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Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NC...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968668/ https://www.ncbi.nlm.nih.gov/pubmed/33730054 http://dx.doi.org/10.1371/journal.pone.0248065 |
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author | Taki, Keigo Takagi, Hiroshi Hirose, Tomonori Sun, Runan Yaginuma, Hiroshi Mizoguchi, Akira Kobayashi, Tomoko Sugiyama, Mariko Tsunekawa, Taku Onoue, Takeshi Hagiwara, Daisuke Ito, Yoshihiro Iwama, Shintaro Suga, Hidetaka Banno, Ryoichi Sakano, Daisuke Kume, Shoen Arima, Hiroshi |
author_facet | Taki, Keigo Takagi, Hiroshi Hirose, Tomonori Sun, Runan Yaginuma, Hiroshi Mizoguchi, Akira Kobayashi, Tomoko Sugiyama, Mariko Tsunekawa, Taku Onoue, Takeshi Hagiwara, Daisuke Ito, Yoshihiro Iwama, Shintaro Suga, Hidetaka Banno, Ryoichi Sakano, Daisuke Kume, Shoen Arima, Hiroshi |
author_sort | Taki, Keigo |
collection | PubMed |
description | Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NCD plus high-NaCl diet (NCD plus NaCl), a HFD, or a HFD plus high-NaCl diet (HFD plus NaCl) for 30 weeks. No significant differences in body weight gain, insulin sensitivity, and glucose tolerance were observed between NCD-fed and NCD plus NaCl-fed mice. In contrast, body and liver weights were decreased, but the weight of epididymal white adipose tissue was increased in HFD plus NaCl-fed compared to HFD-fed mice. HFD plus NaCl-fed mice had lower plasma glucose levels in an insulin tolerance test, and showed higher plasma glucose and lower plasma insulin levels in an intraperitoneal glucose tolerance test compared to HFD-fed mice. The β-cell area and number of islets were decreased in HFD plus NaCl-fed compared to HFD-fed mice. Increased Ki67-positive β-cells, and increased expression levels of Ki67, CyclinB1, and CyclinD1 mRNA in islets were observed in HFD-fed but not HFD plus NaCl-fed mice when compared to NCD-fed mice. Our data suggest that excessive NaCl intake accompanied by a HFD exacerbates glucose intolerance, with impairment in insulin secretion caused by the attenuation of expansion of β-cell mass in the pancreas. |
format | Online Article Text |
id | pubmed-7968668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-79686682021-03-31 Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet Taki, Keigo Takagi, Hiroshi Hirose, Tomonori Sun, Runan Yaginuma, Hiroshi Mizoguchi, Akira Kobayashi, Tomoko Sugiyama, Mariko Tsunekawa, Taku Onoue, Takeshi Hagiwara, Daisuke Ito, Yoshihiro Iwama, Shintaro Suga, Hidetaka Banno, Ryoichi Sakano, Daisuke Kume, Shoen Arima, Hiroshi PLoS One Research Article Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NCD plus high-NaCl diet (NCD plus NaCl), a HFD, or a HFD plus high-NaCl diet (HFD plus NaCl) for 30 weeks. No significant differences in body weight gain, insulin sensitivity, and glucose tolerance were observed between NCD-fed and NCD plus NaCl-fed mice. In contrast, body and liver weights were decreased, but the weight of epididymal white adipose tissue was increased in HFD plus NaCl-fed compared to HFD-fed mice. HFD plus NaCl-fed mice had lower plasma glucose levels in an insulin tolerance test, and showed higher plasma glucose and lower plasma insulin levels in an intraperitoneal glucose tolerance test compared to HFD-fed mice. The β-cell area and number of islets were decreased in HFD plus NaCl-fed compared to HFD-fed mice. Increased Ki67-positive β-cells, and increased expression levels of Ki67, CyclinB1, and CyclinD1 mRNA in islets were observed in HFD-fed but not HFD plus NaCl-fed mice when compared to NCD-fed mice. Our data suggest that excessive NaCl intake accompanied by a HFD exacerbates glucose intolerance, with impairment in insulin secretion caused by the attenuation of expansion of β-cell mass in the pancreas. Public Library of Science 2021-03-17 /pmc/articles/PMC7968668/ /pubmed/33730054 http://dx.doi.org/10.1371/journal.pone.0248065 Text en © 2021 Taki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Taki, Keigo Takagi, Hiroshi Hirose, Tomonori Sun, Runan Yaginuma, Hiroshi Mizoguchi, Akira Kobayashi, Tomoko Sugiyama, Mariko Tsunekawa, Taku Onoue, Takeshi Hagiwara, Daisuke Ito, Yoshihiro Iwama, Shintaro Suga, Hidetaka Banno, Ryoichi Sakano, Daisuke Kume, Shoen Arima, Hiroshi Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet |
title | Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet |
title_full | Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet |
title_fullStr | Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet |
title_full_unstemmed | Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet |
title_short | Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet |
title_sort | dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968668/ https://www.ncbi.nlm.nih.gov/pubmed/33730054 http://dx.doi.org/10.1371/journal.pone.0248065 |
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