Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR–Cas12a gRNA switch

CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease activity. One promising strategy involves linking nuclease activity to a cell's transcriptional state by engineering guide RNAs (gRNAs) to function only after complexing with a ‘trigger’ RNA. However, standar...

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Detalles Bibliográficos
Autores principales: Collins, Scott P, Rostain, William, Liao, Chunyu, Beisel, Chase L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968991/
https://www.ncbi.nlm.nih.gov/pubmed/33619539
http://dx.doi.org/10.1093/nar/gkab100

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968991/
https://www.ncbi.nlm.nih.gov/pubmed/33619539
http://dx.doi.org/10.1093/nar/gkab100

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