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Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR–Cas12a gRNA switch
CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease activity. One promising strategy involves linking nuclease activity to a cell's transcriptional state by engineering guide RNAs (gRNAs) to function only after complexing with a ‘trigger’ RNA. However, standar...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968991/ https://www.ncbi.nlm.nih.gov/pubmed/33619539 http://dx.doi.org/10.1093/nar/gkab100 |