Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency

The CRISPR/Cas9 system is a technology for genome engineering, which has been applied to indel mutations in genes as well as targeted gene deletion and replacement. Here, we describe paired gRNA deletions along the PIGA locus on the human X chromosome ranging from 17 kb to 2 Mb. We found no compelli...

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Detalles Bibliográficos
Autores principales: Li, Donghui, Sun, Xiaoji, Yu, Fangzhou, Perle, Mary Ann, Araten, David, Boeke, Jef D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969003/
https://www.ncbi.nlm.nih.gov/pubmed/33591308
http://dx.doi.org/10.1093/nar/gkab035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969003/
https://www.ncbi.nlm.nih.gov/pubmed/33591308
http://dx.doi.org/10.1093/nar/gkab035

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