Cargando…

Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames

Upstream open reading frame (uORF) translation disrupts scanning 43S flux on mRNA and modulates main open reading frame (mORF) translation efficiency. Current tools, however, have limited access to ribosome dynamics in both upstream and main ORFs of an mRNA. Here, we develop a new two-color in vitro...

Descripción completa

Detalles Bibliográficos
Autores principales: Gaba, Anthony, Wang, Hongyun, Fortune, Trinisia, Qu, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969011/
https://www.ncbi.nlm.nih.gov/pubmed/33330921
http://dx.doi.org/10.1093/nar/gkaa1185