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Meiocyte Isolation by INTACT and Meiotic Transcriptome Analysis in Arabidopsis

Isolation of nuclei tagged in specific cell types (INTACT) is a method developed to isolate cell-type-specific nuclei that are tagged through in vivo biotin labeling of a nuclear targeting fusion (NTF) protein. In our work, INTACT was used to capture nuclei of meiocytes and to generate a meiotic tra...

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Detalles Bibliográficos
Autores principales: Barra, Lucia, Termolino, Pasquale, Aiese Cigliano, Riccardo, Cremona, Gaetana, Paparo, Rosa, Lanzillo, Carmine, Consiglio, Maria Federica, Conicella, Clara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969724/
https://www.ncbi.nlm.nih.gov/pubmed/33747019
http://dx.doi.org/10.3389/fpls.2021.638051