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smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

Recently, advances in fluorescent in-situ hybridization techniques and in imaging technology have enabled visualization and counting of individual RNA molecules in single cells. This has greatly enhanced the resolution in our understanding of transcriptional processes. Here, we adapt a recently publ...

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Detalles Bibliográficos
Autores principales: Calvo, Llilians, Ronshaugen, Matthew, Pettini, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979837/
https://www.ncbi.nlm.nih.gov/pubmed/33742105
http://dx.doi.org/10.1038/s42003-021-01803-0