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Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
GFP fusion-based fluorescence-detection size-exclusion chromatography (FSEC) has been widely employed for membrane protein expression screening. However, fused GFP itself may occasionally affect the expression and/or stability of the targeted membrane protein, leading to both false-positive and fals...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979870/ https://www.ncbi.nlm.nih.gov/pubmed/33742097 http://dx.doi.org/10.1038/s42003-021-01891-y |