Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines

Major histocompatibility complexes (MHC) play a critical role in immunity by presenting peptides on the cell surface for T cell recognition. Identification of these peptides can be valuable to develop vaccines or immunotherapeutic strategies for infectious diseases and cancers. Mass spectrometry is...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohan, Sonali V., Datta, Keshava K., Ziegman, Rebekah, Smith, Corey, Gowda, Harsha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982746/
https://www.ncbi.nlm.nih.gov/pubmed/33778779
http://dx.doi.org/10.1016/j.xpro.2021.100385
_version_ 1783667784021966848
author Mohan, Sonali V.
Datta, Keshava K.
Ziegman, Rebekah
Smith, Corey
Gowda, Harsha
author_facet Mohan, Sonali V.
Datta, Keshava K.
Ziegman, Rebekah
Smith, Corey
Gowda, Harsha
author_sort Mohan, Sonali V.
collection PubMed
description Major histocompatibility complexes (MHC) play a critical role in immunity by presenting peptides on the cell surface for T cell recognition. Identification of these peptides can be valuable to develop vaccines or immunotherapeutic strategies for infectious diseases and cancers. Mass spectrometry is the only tool available for unbiased identification of the immunopeptidome. Here, we describe a protocol for purification and identification of MHC class I peptides, including in-house purification of anti-MHC-antibody from hybridoma cells and the LC-MS/MS analysis of MHC-I bound peptides.
format Online
Article
Text
id pubmed-7982746
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-79827462021-03-25 Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines Mohan, Sonali V. Datta, Keshava K. Ziegman, Rebekah Smith, Corey Gowda, Harsha STAR Protoc Protocol Major histocompatibility complexes (MHC) play a critical role in immunity by presenting peptides on the cell surface for T cell recognition. Identification of these peptides can be valuable to develop vaccines or immunotherapeutic strategies for infectious diseases and cancers. Mass spectrometry is the only tool available for unbiased identification of the immunopeptidome. Here, we describe a protocol for purification and identification of MHC class I peptides, including in-house purification of anti-MHC-antibody from hybridoma cells and the LC-MS/MS analysis of MHC-I bound peptides. Elsevier 2021-03-18 /pmc/articles/PMC7982746/ /pubmed/33778779 http://dx.doi.org/10.1016/j.xpro.2021.100385 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Mohan, Sonali V.
Datta, Keshava K.
Ziegman, Rebekah
Smith, Corey
Gowda, Harsha
Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_full Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_fullStr Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_full_unstemmed Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_short Protocol for purification and identification of MHC class I immunopeptidome from cancer cell lines
title_sort protocol for purification and identification of mhc class i immunopeptidome from cancer cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982746/
https://www.ncbi.nlm.nih.gov/pubmed/33778779
http://dx.doi.org/10.1016/j.xpro.2021.100385
work_keys_str_mv AT mohansonaliv protocolforpurificationandidentificationofmhcclassiimmunopeptidomefromcancercelllines
AT dattakeshavak protocolforpurificationandidentificationofmhcclassiimmunopeptidomefromcancercelllines
AT ziegmanrebekah protocolforpurificationandidentificationofmhcclassiimmunopeptidomefromcancercelllines
AT smithcorey protocolforpurificationandidentificationofmhcclassiimmunopeptidomefromcancercelllines
AT gowdaharsha protocolforpurificationandidentificationofmhcclassiimmunopeptidomefromcancercelllines

Ejemplares similares