Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds

Controversies remain over the standard procedures for the modeling of skin fibrosis and its use in in vitro testing of different drugs. Here, we report a reproducible protocol for producing a skin fibrosis model using human dermal fibroblasts seeded in collagen hydrogel. Detailed procedures for the...

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Detalles Bibliográficos
Autores principales: Alsharabasy, Amir M., Pandit, Abhay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982747/
https://www.ncbi.nlm.nih.gov/pubmed/33778781
http://dx.doi.org/10.1016/j.xpro.2021.100387
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author Alsharabasy, Amir M.
Pandit, Abhay
author_facet Alsharabasy, Amir M.
Pandit, Abhay
author_sort Alsharabasy, Amir M.
collection PubMed
description Controversies remain over the standard procedures for the modeling of skin fibrosis and its use in in vitro testing of different drugs. Here, we report a reproducible protocol for producing a skin fibrosis model using human dermal fibroblasts seeded in collagen hydrogel. Detailed procedures for the fabrication of cell/hydrogel constructs, fibrosis induction, protein extraction for western blotting analysis are presented along with how this model can be employed for investigating the possible anti-fibrotic functions of certain chemical compounds.
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spelling pubmed-79827472021-03-25 Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds Alsharabasy, Amir M. Pandit, Abhay STAR Protoc Protocol Controversies remain over the standard procedures for the modeling of skin fibrosis and its use in in vitro testing of different drugs. Here, we report a reproducible protocol for producing a skin fibrosis model using human dermal fibroblasts seeded in collagen hydrogel. Detailed procedures for the fabrication of cell/hydrogel constructs, fibrosis induction, protein extraction for western blotting analysis are presented along with how this model can be employed for investigating the possible anti-fibrotic functions of certain chemical compounds. Elsevier 2021-03-18 /pmc/articles/PMC7982747/ /pubmed/33778781 http://dx.doi.org/10.1016/j.xpro.2021.100387 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Alsharabasy, Amir M.
Pandit, Abhay
Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds
title Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds
title_full Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds
title_fullStr Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds
title_full_unstemmed Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds
title_short Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds
title_sort protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982747/
https://www.ncbi.nlm.nih.gov/pubmed/33778781
http://dx.doi.org/10.1016/j.xpro.2021.100387
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