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Purification of micronuclei from cultured cells by flow cytometry
Micronuclei are aberrant nuclear compartments that form when chromosomes or chromosome fragments fail to incorporate into a primary nucleus during mitotic exit. Ruptures at the micronuclear envelope are associated with DNA damage and activation of immune sensing pathways. To gain insights into these...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982749/ https://www.ncbi.nlm.nih.gov/pubmed/33778777 http://dx.doi.org/10.1016/j.xpro.2021.100378 |
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author | Toufektchan, Eléonore Maciejowski, John |
author_facet | Toufektchan, Eléonore Maciejowski, John |
author_sort | Toufektchan, Eléonore |
collection | PubMed |
description | Micronuclei are aberrant nuclear compartments that form when chromosomes or chromosome fragments fail to incorporate into a primary nucleus during mitotic exit. Ruptures at the micronuclear envelope are associated with DNA damage and activation of immune sensing pathways. To gain insights into these processes, we have developed a method to purify ruptured micronuclei. This method paves the way toward understanding the consequences of micronuclear envelope rupture. For complete details on the use and execution of this protocol, please refer to Mohr et al. (2021). |
format | Online Article Text |
id | pubmed-7982749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-79827492021-03-25 Purification of micronuclei from cultured cells by flow cytometry Toufektchan, Eléonore Maciejowski, John STAR Protoc Protocol Micronuclei are aberrant nuclear compartments that form when chromosomes or chromosome fragments fail to incorporate into a primary nucleus during mitotic exit. Ruptures at the micronuclear envelope are associated with DNA damage and activation of immune sensing pathways. To gain insights into these processes, we have developed a method to purify ruptured micronuclei. This method paves the way toward understanding the consequences of micronuclear envelope rupture. For complete details on the use and execution of this protocol, please refer to Mohr et al. (2021). Elsevier 2021-03-18 /pmc/articles/PMC7982749/ /pubmed/33778777 http://dx.doi.org/10.1016/j.xpro.2021.100378 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Toufektchan, Eléonore Maciejowski, John Purification of micronuclei from cultured cells by flow cytometry |
title | Purification of micronuclei from cultured cells by flow cytometry |
title_full | Purification of micronuclei from cultured cells by flow cytometry |
title_fullStr | Purification of micronuclei from cultured cells by flow cytometry |
title_full_unstemmed | Purification of micronuclei from cultured cells by flow cytometry |
title_short | Purification of micronuclei from cultured cells by flow cytometry |
title_sort | purification of micronuclei from cultured cells by flow cytometry |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982749/ https://www.ncbi.nlm.nih.gov/pubmed/33778777 http://dx.doi.org/10.1016/j.xpro.2021.100378 |
work_keys_str_mv | AT toufektchaneleonore purificationofmicronucleifromculturedcellsbyflowcytometry AT maciejowskijohn purificationofmicronucleifromculturedcellsbyflowcytometry |