Live imaging and quantitation of nascent transcription using the MS2/MCP system in the Drosophila embryo

Visualizing transcription live in Drosophila is providing important new insights into the spatiotemporal regulation of transcription. Here, we describe a protocol to visualize and quantitate transcription from gene loci that are tagged with MS2 stem-loop sequences in the Drosophila embryo. MS2 stem-...

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Detalles Bibliográficos
Autores principales: Hoppe, Caroline, Ashe, Hilary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982776/
https://www.ncbi.nlm.nih.gov/pubmed/33778778
http://dx.doi.org/10.1016/j.xpro.2021.100379

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982776/
https://www.ncbi.nlm.nih.gov/pubmed/33778778
http://dx.doi.org/10.1016/j.xpro.2021.100379

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