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Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes

Five established clearing protocols were compared with a modified and simplified method to determine an optimal clearing reagent for three-dimensionally visualizing fluorophores in the murine liver, a challenging organ to clear. We report successful clearing of whole liver lobes by modification of a...

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Detalles Bibliográficos
Autores principales: Hough, Michelle, Fenlon, Michael, Glazier, Alison, Short, Celia, Fernandez, Gerardo Esteban, Xu, Jiabo, Mahdi, Elaa, Asahina, Kinji, Wang, Kasper S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983039/
https://www.ncbi.nlm.nih.gov/pubmed/33467918
http://dx.doi.org/10.2144/btn-2020-0063