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Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes
Five established clearing protocols were compared with a modified and simplified method to determine an optimal clearing reagent for three-dimensionally visualizing fluorophores in the murine liver, a challenging organ to clear. We report successful clearing of whole liver lobes by modification of a...
Autores principales: | Hough, Michelle, Fenlon, Michael, Glazier, Alison, Short, Celia, Fernandez, Gerardo Esteban, Xu, Jiabo, Mahdi, Elaa, Asahina, Kinji, Wang, Kasper S |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983039/ https://www.ncbi.nlm.nih.gov/pubmed/33467918 http://dx.doi.org/10.2144/btn-2020-0063 |
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