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A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies

Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the sp...

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Autores principales: Yao, Zhong, Drecun, Luka, Aboualizadeh, Farzaneh, Kim, Sun Jin, Li, Zhijie, Wood, Heidi, Valcourt, Emelissa J., Manguiat, Kathy, Plenderleith, Simon, Yip, Lily, Li, Xinliu, Zhong, Zoe, Yue, Feng Yun, Closas, Tatiana, Snider, Jamie, Tomic, Jelena, Drews, Steven J., Drebot, Michael A., McGeer, Allison, Ostrowski, Mario, Mubareka, Samira, Rini, James M., Owen, Shawn, Stagljar, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985487/
https://www.ncbi.nlm.nih.gov/pubmed/33753733
http://dx.doi.org/10.1038/s41467-021-22102-6
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author Yao, Zhong
Drecun, Luka
Aboualizadeh, Farzaneh
Kim, Sun Jin
Li, Zhijie
Wood, Heidi
Valcourt, Emelissa J.
Manguiat, Kathy
Plenderleith, Simon
Yip, Lily
Li, Xinliu
Zhong, Zoe
Yue, Feng Yun
Closas, Tatiana
Snider, Jamie
Tomic, Jelena
Drews, Steven J.
Drebot, Michael A.
McGeer, Allison
Ostrowski, Mario
Mubareka, Samira
Rini, James M.
Owen, Shawn
Stagljar, Igor
author_facet Yao, Zhong
Drecun, Luka
Aboualizadeh, Farzaneh
Kim, Sun Jin
Li, Zhijie
Wood, Heidi
Valcourt, Emelissa J.
Manguiat, Kathy
Plenderleith, Simon
Yip, Lily
Li, Xinliu
Zhong, Zoe
Yue, Feng Yun
Closas, Tatiana
Snider, Jamie
Tomic, Jelena
Drews, Steven J.
Drebot, Michael A.
McGeer, Allison
Ostrowski, Mario
Mubareka, Samira
Rini, James M.
Owen, Shawn
Stagljar, Igor
author_sort Yao, Zhong
collection PubMed
description Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELISA, and its readouts are consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics, as well as disease and vaccination management.
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spelling pubmed-79854872021-04-16 A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies Yao, Zhong Drecun, Luka Aboualizadeh, Farzaneh Kim, Sun Jin Li, Zhijie Wood, Heidi Valcourt, Emelissa J. Manguiat, Kathy Plenderleith, Simon Yip, Lily Li, Xinliu Zhong, Zoe Yue, Feng Yun Closas, Tatiana Snider, Jamie Tomic, Jelena Drews, Steven J. Drebot, Michael A. McGeer, Allison Ostrowski, Mario Mubareka, Samira Rini, James M. Owen, Shawn Stagljar, Igor Nat Commun Article Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELISA, and its readouts are consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics, as well as disease and vaccination management. Nature Publishing Group UK 2021-03-22 /pmc/articles/PMC7985487/ /pubmed/33753733 http://dx.doi.org/10.1038/s41467-021-22102-6 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yao, Zhong
Drecun, Luka
Aboualizadeh, Farzaneh
Kim, Sun Jin
Li, Zhijie
Wood, Heidi
Valcourt, Emelissa J.
Manguiat, Kathy
Plenderleith, Simon
Yip, Lily
Li, Xinliu
Zhong, Zoe
Yue, Feng Yun
Closas, Tatiana
Snider, Jamie
Tomic, Jelena
Drews, Steven J.
Drebot, Michael A.
McGeer, Allison
Ostrowski, Mario
Mubareka, Samira
Rini, James M.
Owen, Shawn
Stagljar, Igor
A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
title A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
title_full A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
title_fullStr A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
title_full_unstemmed A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
title_short A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
title_sort homogeneous split-luciferase assay for rapid and sensitive detection of anti-sars cov-2 antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985487/
https://www.ncbi.nlm.nih.gov/pubmed/33753733
http://dx.doi.org/10.1038/s41467-021-22102-6
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