Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells

The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humoral immune responses. Here, we describe a protocol for the sequencing, cloning, and characterization of antibody genes that encode BCRs. We used this method to analyze the BCRs of different mouse B cell popula...

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Detalles Bibliográficos
Autores principales: Viant, Charlotte, Escolano, Amelia, Chen, Spencer T., Nussenzweig, Michel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985702/
https://www.ncbi.nlm.nih.gov/pubmed/33778783
http://dx.doi.org/10.1016/j.xpro.2021.100389

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985702/
https://www.ncbi.nlm.nih.gov/pubmed/33778783
http://dx.doi.org/10.1016/j.xpro.2021.100389

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