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Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells
The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humoral immune responses. Here, we describe a protocol for the sequencing, cloning, and characterization of antibody genes that encode BCRs. We used this method to analyze the BCRs of different mouse B cell popula...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985702/ https://www.ncbi.nlm.nih.gov/pubmed/33778783 http://dx.doi.org/10.1016/j.xpro.2021.100389 |