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Linear flow‐velocity gradient chromatography—An efficient method for increasing the process efficiency of batch and continuous capture chromatography of proteins

A new method was proposed for increasing the capture chromatography process efficiency, linear flow‐velocity gradient (LFG). The method uses a linear decreasing flow‐velocity gradient with time during the sample loading. The initial flow velocity, the final flow velocity and the gradient time are th...

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Detalles Bibliográficos
Autores principales: Chen, Chyi‐Shin, Ando, Kosei, Yoshimoto, Noriko, Yamamoto, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986079/
https://www.ncbi.nlm.nih.gov/pubmed/33283261
http://dx.doi.org/10.1002/bit.27649