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Linear flow‐velocity gradient chromatography—An efficient method for increasing the process efficiency of batch and continuous capture chromatography of proteins
A new method was proposed for increasing the capture chromatography process efficiency, linear flow‐velocity gradient (LFG). The method uses a linear decreasing flow‐velocity gradient with time during the sample loading. The initial flow velocity, the final flow velocity and the gradient time are th...
Autores principales: | Chen, Chyi‐Shin, Ando, Kosei, Yoshimoto, Noriko, Yamamoto, Shuichi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986079/ https://www.ncbi.nlm.nih.gov/pubmed/33283261 http://dx.doi.org/10.1002/bit.27649 |
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