A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast

BACKGROUND: CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene. Effective, inducible CRISPRi has been demonstrated in budding yeast, and genome-scale guide libraries enable systematic, genome-wide...

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Detalles Bibliográficos
Autores principales: McGlincy, Nicholas J., Meacham, Zuriah A., Reynaud, Kendra K., Muller, Ryan, Baum, Rachel, Ingolia, Nicholas T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986282/
https://www.ncbi.nlm.nih.gov/pubmed/33757429
http://dx.doi.org/10.1186/s12864-021-07518-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986282/
https://www.ncbi.nlm.nih.gov/pubmed/33757429
http://dx.doi.org/10.1186/s12864-021-07518-0

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