Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species

Understanding how SARS-CoV-2 interacts with different mammalian angiotensin-converting enzyme II (ACE2) cell entry receptors elucidates determinants of virus transmission and facilitates development of vaccines for humans and animals. Yeast display-based directed evolution identified conserved ACE2...

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Detalles Bibliográficos
Autores principales: Heinzelman, Pete, Greenhalgh, Jonathan C., Romero, Philip A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987019/
https://www.ncbi.nlm.nih.gov/pubmed/33758860
http://dx.doi.org/10.1101/2021.03.16.435705
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author Heinzelman, Pete
Greenhalgh, Jonathan C.
Romero, Philip A.
author_facet Heinzelman, Pete
Greenhalgh, Jonathan C.
Romero, Philip A.
author_sort Heinzelman, Pete
collection PubMed
description Understanding how SARS-CoV-2 interacts with different mammalian angiotensin-converting enzyme II (ACE2) cell entry receptors elucidates determinants of virus transmission and facilitates development of vaccines for humans and animals. Yeast display-based directed evolution identified conserved ACE2 mutations that increase spike binding across multiple species. Gln42Leu increased ACE2-spike binding for human and four of four other mammalian ACE2s; Leu79Ile had a effect for human and three of three mammalian ACE2s. These residues are highly represented, 83% for Gln42 and 56% for Leu79, among mammalian ACE2s. The above findings can be important in protecting humans and animals from existing and future SARS-CoV-2 variants.
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spelling pubmed-79870192021-03-24 Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species Heinzelman, Pete Greenhalgh, Jonathan C. Romero, Philip A. bioRxiv Article Understanding how SARS-CoV-2 interacts with different mammalian angiotensin-converting enzyme II (ACE2) cell entry receptors elucidates determinants of virus transmission and facilitates development of vaccines for humans and animals. Yeast display-based directed evolution identified conserved ACE2 mutations that increase spike binding across multiple species. Gln42Leu increased ACE2-spike binding for human and four of four other mammalian ACE2s; Leu79Ile had a effect for human and three of three mammalian ACE2s. These residues are highly represented, 83% for Gln42 and 56% for Leu79, among mammalian ACE2s. The above findings can be important in protecting humans and animals from existing and future SARS-CoV-2 variants. Cold Spring Harbor Laboratory 2022-01-04 /pmc/articles/PMC7987019/ /pubmed/33758860 http://dx.doi.org/10.1101/2021.03.16.435705 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Heinzelman, Pete
Greenhalgh, Jonathan C.
Romero, Philip A.
Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
title Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
title_full Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
title_fullStr Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
title_full_unstemmed Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
title_short Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
title_sort yeast surface display-based identification of ace2 mutations that modulate sars-cov-2 spike binding across multiple mammalian species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987019/
https://www.ncbi.nlm.nih.gov/pubmed/33758860
http://dx.doi.org/10.1101/2021.03.16.435705
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