Functional proteomics protocol for the identification of interaction partners in Tetrahymena thermophila

We describe an optimized protocol for one-step affinity purification of FZZ-tagged proteins followed by mass spectrometry analysis for the identification of protein-protein interactions in the ciliate protozoan Tetrahymena thermophila. The FZZ epitope tag contains 2 protein A moieties (ZZ) and a 3xF...

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Detalles Bibliográficos
Autores principales: Nabeel-Shah, Syed, Garg, Jyoti, Kougnassoukou Tchara, Pata-Eting, Pearlman, Ronald E., Lambert, Jean-Philippe, Fillingham, Jeffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988224/
https://www.ncbi.nlm.nih.gov/pubmed/33786459
http://dx.doi.org/10.1016/j.xpro.2021.100362

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988224/
https://www.ncbi.nlm.nih.gov/pubmed/33786459
http://dx.doi.org/10.1016/j.xpro.2021.100362

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