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CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriopha...

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Detalles Bibliográficos
Autores principales: Wetzel, Katherine S., Guerrero-Bustamante, Carlos A., Dedrick, Rebekah M., Ko, Ching-Chung, Freeman, Krista G., Aull, Haley G., Divens, Ashley M., Rock, Jeremy M., Zack, Kira M., Hatfull, Graham F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990910/
https://www.ncbi.nlm.nih.gov/pubmed/33762639
http://dx.doi.org/10.1038/s41598-021-86112-6