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CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriopha...
Autores principales: | Wetzel, Katherine S., Guerrero-Bustamante, Carlos A., Dedrick, Rebekah M., Ko, Ching-Chung, Freeman, Krista G., Aull, Haley G., Divens, Ashley M., Rock, Jeremy M., Zack, Kira M., Hatfull, Graham F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990910/ https://www.ncbi.nlm.nih.gov/pubmed/33762639 http://dx.doi.org/10.1038/s41598-021-86112-6 |
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