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RNA silencing suppressor-influenced performance of a virus vector delivering both guide RNA and Cas9 for CRISPR gene editing

We report on further development of the agroinfiltratable Tobacco mosaic virus (TMV)-based overexpression (TRBO) vector to deliver CRISPR/Cas9 components into plants. First, production of a Cas9 (HcoCas9) protein from a binary plasmid increased when co-expressed in presence of suppressors of gene si...

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Detalles Bibliográficos
Autores principales: Chiong, Kelvin T., Cody, Will B., Scholthof, Herman B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990971/
https://www.ncbi.nlm.nih.gov/pubmed/33762584
http://dx.doi.org/10.1038/s41598-021-85366-4