Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability

Our previous study found that lncRNA gadd7 was up-regulated in the semen of varicocele patients, and could promote the apoptosis of mouse spermatocytes and inhibit their proliferation. Therefore, we further explored whether down-regulation of Gadd seven expression could protect the viability of sper...

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Autores principales: Zhao, Jun, Ma, Wenmin, Zhong, Yucheng, Deng, Hao, Zhou, Bingyu, Wu, Yaqin, Yang, Meiqiong, Li, Huan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991575/
https://www.ncbi.nlm.nih.gov/pubmed/33778010
http://dx.doi.org/10.3389/fmolb.2021.652392
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author Zhao, Jun
Ma, Wenmin
Zhong, Yucheng
Deng, Hao
Zhou, Bingyu
Wu, Yaqin
Yang, Meiqiong
Li, Huan
author_facet Zhao, Jun
Ma, Wenmin
Zhong, Yucheng
Deng, Hao
Zhou, Bingyu
Wu, Yaqin
Yang, Meiqiong
Li, Huan
author_sort Zhao, Jun
collection PubMed
description Our previous study found that lncRNA gadd7 was up-regulated in the semen of varicocele patients, and could promote the apoptosis of mouse spermatocytes and inhibit their proliferation. Therefore, we further explored whether down-regulation of Gadd seven expression could protect the viability of spermatocytes. Here we designed specific sgRNAs targeting the ORF region of gadd7, and constructed a CRISPR-dCas9-KRAB system that effectively inhibits gadd7 at the transcriptional level. The CRISPRi system can effectively prevent the apoptosis of spermatocytes and enhance their proliferation, which is expected to provide a potentially effective molecular intervention method for the treatment of male infertility caused by varicocele.
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spelling pubmed-79915752021-03-26 Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability Zhao, Jun Ma, Wenmin Zhong, Yucheng Deng, Hao Zhou, Bingyu Wu, Yaqin Yang, Meiqiong Li, Huan Front Mol Biosci Molecular Biosciences Our previous study found that lncRNA gadd7 was up-regulated in the semen of varicocele patients, and could promote the apoptosis of mouse spermatocytes and inhibit their proliferation. Therefore, we further explored whether down-regulation of Gadd seven expression could protect the viability of spermatocytes. Here we designed specific sgRNAs targeting the ORF region of gadd7, and constructed a CRISPR-dCas9-KRAB system that effectively inhibits gadd7 at the transcriptional level. The CRISPRi system can effectively prevent the apoptosis of spermatocytes and enhance their proliferation, which is expected to provide a potentially effective molecular intervention method for the treatment of male infertility caused by varicocele. Frontiers Media S.A. 2021-03-11 /pmc/articles/PMC7991575/ /pubmed/33778010 http://dx.doi.org/10.3389/fmolb.2021.652392 Text en Copyright © 2021 Zhao, Ma, Zhong, Deng, Zhou, Wu, Yang and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Zhao, Jun
Ma, Wenmin
Zhong, Yucheng
Deng, Hao
Zhou, Bingyu
Wu, Yaqin
Yang, Meiqiong
Li, Huan
Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability
title Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability
title_full Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability
title_fullStr Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability
title_full_unstemmed Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability
title_short Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability
title_sort transcriptional inhibition of lncrna gadd7 by crispr/dcas9-krab protects spermatocyte viability
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991575/
https://www.ncbi.nlm.nih.gov/pubmed/33778010
http://dx.doi.org/10.3389/fmolb.2021.652392
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