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K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology

BACKGROUND: K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is presented. The protocol was accompanied by software developed to aid with primer set...

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Detalles Bibliográficos
Autores principales: Ziarsolo, Peio, Hasing, Tomas, Hilario, Rebeca, Garcia-Carpintero, Victor, Blanca, Jose, Bombarely, Aureliano, Cañizares, Joaquin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993484/
https://www.ncbi.nlm.nih.gov/pubmed/33766048
http://dx.doi.org/10.1186/s13007-021-00733-6