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K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology
BACKGROUND: K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is presented. The protocol was accompanied by software developed to aid with primer set...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993484/ https://www.ncbi.nlm.nih.gov/pubmed/33766048 http://dx.doi.org/10.1186/s13007-021-00733-6 |