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Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice
Jak3 is the only non-promiscuous member of the Jak family of secondary messengers. Studies to date have focused on understanding and targeting the cell-autonomous role of Jak3 in immunity, while functional Jak3 expression outside the hematopoietic system remains largely unreported. We show that Jak3...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994450/ https://www.ncbi.nlm.nih.gov/pubmed/33767339 http://dx.doi.org/10.1038/s42003-021-01846-3 |
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author | Barcia Durán, José Gabriel Lu, Tyler Houghton, Sean Geng, Fuqiang Schreiner, Ryan Xiang, Jenny Rafii, Shahin Redmond, David Lis, Raphaël |
author_facet | Barcia Durán, José Gabriel Lu, Tyler Houghton, Sean Geng, Fuqiang Schreiner, Ryan Xiang, Jenny Rafii, Shahin Redmond, David Lis, Raphaël |
author_sort | Barcia Durán, José Gabriel |
collection | PubMed |
description | Jak3 is the only non-promiscuous member of the Jak family of secondary messengers. Studies to date have focused on understanding and targeting the cell-autonomous role of Jak3 in immunity, while functional Jak3 expression outside the hematopoietic system remains largely unreported. We show that Jak3 is expressed in endothelial cells across hematopoietic and non-hematopoietic organs, with heightened expression in the bone marrow. The bone marrow niche is understood as a network of different cell types that regulate hematopoietic function. We show that the Jak3(–/–) bone marrow niche is deleterious for the maintenance of long-term repopulating hematopoietic stem cells (LT-HSCs) and that JAK3-overexpressing endothelial cells have increased potential to expand LT-HSCs in vitro. This work may serve to identify a novel function for a highly specific tyrosine kinase in the bone marrow vascular niche and to further characterize the LT-HSC function of sinusoidal endothelium. |
format | Online Article Text |
id | pubmed-7994450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79944502021-04-16 Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice Barcia Durán, José Gabriel Lu, Tyler Houghton, Sean Geng, Fuqiang Schreiner, Ryan Xiang, Jenny Rafii, Shahin Redmond, David Lis, Raphaël Commun Biol Article Jak3 is the only non-promiscuous member of the Jak family of secondary messengers. Studies to date have focused on understanding and targeting the cell-autonomous role of Jak3 in immunity, while functional Jak3 expression outside the hematopoietic system remains largely unreported. We show that Jak3 is expressed in endothelial cells across hematopoietic and non-hematopoietic organs, with heightened expression in the bone marrow. The bone marrow niche is understood as a network of different cell types that regulate hematopoietic function. We show that the Jak3(–/–) bone marrow niche is deleterious for the maintenance of long-term repopulating hematopoietic stem cells (LT-HSCs) and that JAK3-overexpressing endothelial cells have increased potential to expand LT-HSCs in vitro. This work may serve to identify a novel function for a highly specific tyrosine kinase in the bone marrow vascular niche and to further characterize the LT-HSC function of sinusoidal endothelium. Nature Publishing Group UK 2021-03-25 /pmc/articles/PMC7994450/ /pubmed/33767339 http://dx.doi.org/10.1038/s42003-021-01846-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Barcia Durán, José Gabriel Lu, Tyler Houghton, Sean Geng, Fuqiang Schreiner, Ryan Xiang, Jenny Rafii, Shahin Redmond, David Lis, Raphaël Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice |
title | Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice |
title_full | Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice |
title_fullStr | Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice |
title_full_unstemmed | Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice |
title_short | Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice |
title_sort | endothelial jak3 expression enhances pro-hematopoietic angiocrine function in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994450/ https://www.ncbi.nlm.nih.gov/pubmed/33767339 http://dx.doi.org/10.1038/s42003-021-01846-3 |
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